摘要
Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.
Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.
出处
《云南植物研究》
CSCD
北大核心
2002年第5期656-658,共3页
Acta Botanica Yunnanica
关键词
根癌农杆菌
转化
紫草
冠瘿组织培养
红色株系
Lithospermum erythrorhizon
Agrobacterium tumefaciens
Crown Gall Tissue Culture
Shikonin .
