化学刺激或电刺激大鼠穹窿下器官诱发的饮水行为和脑内c-fos表达

DRINKING BEHAVIOR AND c-fos EXPRESSION INDUCED BY CHEMICAL OR ELECTRICAL STIMULATION OF SFO IN RAT BRAIN

目的 :对化学刺激和电刺激穹窿下器官 (subfornicalorgan ,SFO)诱发的饮水量和脑内c fos表达的结果是否不同进行比较。方法 :向大鼠SFO内微量注射L 谷氨酸作为化学刺激 ,用恒流刺激SFO作为电刺激 ,记录诱发的 1h内饮水量 ,用免疫组化方法检测脑内Fos蛋白表达。结果 :电刺激和化学刺激SFO均能诱导相似的饮水行为 ,其诱饮率分别为 75 %和 85 .7% ,1h平均饮水量分别为 (0 .2 8± 0 .2 2 )ml和 (0 .31± 0 .15 )ml ,明显高于各自的对照组 (P <0 .0 5 ) ,并均能使前脑的 11个脑区 (终板血管器官 ,正中视前核 ,室旁核 ,视上核 ,下丘脑外侧区 ,丘脑室旁核 ,联合核和中央内侧核 ,终纹床核 ,穹窿周背区和无名质 )和后脑的 4个脑区 (最后区 ,孤束核 ,臂旁外侧核和中缝背核 )相似的Fos蛋白表达。结论 :刺激SFO所诱导的饮水行为和脑内Fos蛋白表达是激活其神经元胞体的结果。

Aim: To compare the drinking behavior and c fos expression induced by chemical or electrical stimulation of subfornical organ (SFO) in rat brain. Methods: L glutamic acid microinjection and constant electrical current were used as chemical and electrical stimulation of SFO, respectively. The water intake over 1 h was recorded and Fos expression was examined immunohistochemically. Results: A similar volume of water intake and Fos expression pattern were induced by both methods of stimulation of SFO. These include 11 forebrain areas (organum vasculosum of the lamina terminalis, median preoptic nucleus, hypothalamic paraventricular nucleus, supraoptic nucleus and lateral hypothalamic area, paraventricular nucleus, reuniens nucleus and central medial nucleus of thalamus, bed nucleus of the stria terminalis, perifornical dorsal area and substantia innominata) and 4 areas of hindbrain (area postrema, nucleus solitary tract, lateral parabrachial nucleus and dorsal raphe nucleus). Conclusion: The drinking behavior and Fos expression in brain induced by SFO stimulation are the results of activation of the neuronal bodies in SFO.