下丘脑背内侧核在缰核兴奋诱发的心血管反应及腓深神经抑制中的作用

THE ROLE OF DORSOMEDIAL HYPOTHALAMIC NUCLEUS IN THE CARDIOVASCULAR RESPONSES INDUCED BY HABENULAR NUCLEUS STIMULATION AND INHIBITORY EFFECT OF DEEP PERONEAL NERVE

目的 :阐明下丘脑背内侧核 (DMH)在缰核 (Hb)兴奋诱发的心血管反应中的作用及DMH在腓深神经 (DPN)传入冲动调节Hb兴奋诱发的心血管活动中的作用及机制。方法 :脲酯和氯醛糖混合静脉麻醉的家兔 ,电刺激Hb、腓深神经 ,记录股动脉血压及心外膜电图 ,DMH内微量注射受体拮抗剂。结果 :同侧DMH微量注射谷氨酸受体阻断剂Kynurenicacid ,部分取消了电刺激Hb兴奋诱发的升压反应及缺血性心电变化反应。同侧DMH微量注射纳洛酮对腓深神经传入冲动抑制Hb兴奋诱发的上述反应有削弱作用。结论 :DMH及其中的谷氨酸受体参与电刺激缰核兴奋诱发的心血管反应 。

Aim:To explore whether dorsomedial hypothalamic nucleus(DMH) is involved in the cardiovascular responses induced by habenular nucleus(Hb) stimulation and inhibitory effect of deep peroneal nerve(DPN) stimulation on above responses or not and to analysis transmitter mechanism. Methods:Experiments were performed on white male rabbits anesthetised with chloralose and urethan .Experimental data were collected by means of stimulating Hb and DPN,recording arterial blood pressure and ectal electrocardiogram (EECG) and microinjecting transmitter blocker. Results Promininet pressor and ischemic EECG segment changes were elicited by Hb stimulation( P <0.01) Microinjecting kynurenic acid into ipsilateral DMH had partial blocking effect on pressor and ischemic EECG ST segment changes induced by Hb stimulation ( P <0.01).Stimulation of bilateral DPN prominently inhibited the pressor and ischemic EECG ST changes induced by Hb stimulation( P <0.01, P <0.05).Microinjecting naloxone into ipsilaleral DMH decreased the inhibitory effect of DPN stimulation on above cardiovascular responses elicited by Hb stimulation( P <0.01, P <0.05). Conclution: Glutamic acid receptor in DMH is involved in pressor and ischemic changes induced by Hb stimulation. DMH and opiate peptide receptors in DMH are involved in the inhibitory effect of DPN stimulation on pressor and ischemic changes induced by Hb stimulation.