抗赤霉病小麦地方品种的贮藏蛋白分析

Storage Proteins Analysis of Local Wheat Varieties with High Resistance to Scab

采用A-PAGE和SDS-PAGE方法,对来自不同地方的23个抗赤霉病小麦地方品种的醇溶蛋白和谷蛋白亚基进行了分析。结果表明,在A-PAGE电泳分析中,23个供试品种具有23种不同的醇溶蛋白带型,共分离出39条相对迁移率不同的谱带,其中31条具有多态性,占86.8％,每份材料可电泳出14～23条带,存在着广泛的等位基因变异。在SDS-PAGE电泳分析中,出现了9种不同的高分子量谷蛋白亚基及6种亚基组合类型,优质亚基及亚基组合所占的比例较少,品质评分较低,其变幅为5～9分,平均为6.8分。

Using A-PAGE and SDS-PAGE methods, the gliadin and HMW-glutenin subunits of 23 local wheat varieties with high resistance to scab were analyzed. There were 23 gliadin genotypes in the 23 cultivars. A total of 39 gliadin bands with different relative mobility were observed, while 31 bands (86. 8%) were polymorphic. Each cultivar could separate 14 to 23 bands. There were 9 subunits and 6 genotypes of HMW-GS in 23 cultivars. The quality scores of these cultivars were quite low, ranging from 2 to 9, with the mean of 6. 0.