锶磷灰石体外细胞毒性研究

In vitro Study on the Cytotoxicity of Strontium Substituted Hydroxyapatite

目的评价锶磷灰石的细胞毒性。方法用增殖度法、MTT比色法、流式细胞仪法评价锶磷灰石的细胞毒性,锶磷灰石掺锶量分别为0%、1%、5%、10%、100%。结果所有不同掺锶量锶磷灰石均无明显细胞毒性表现,但随着掺锶量的增加细胞毒性有逐渐明显的趋势。流式细胞仪检测表明,1%、5%锶磷灰石的细胞毒性与纯羟磷灰石无明显差异。结论锶磷灰石具有良好的细胞亲和性;掺锶量为10%、100%的锶磷灰石具有弱细胞毒性。

Objective To evaluate the cytotoxicity of strontium substituted hydroxyapatite.Methods Cell Relative Growth Rate(RGR)method,MTT assay and Flow Cytometry(FCM)method were used,and the strontium substituted hydroxyapatite contained different strontium concentration(0%,1%,5%,10%,100%).Results It was found that there's no apparent cytotoxicity of all the strontium substituted hydroxyapatite,but the cytotoxicity increased as the strontium concentration raised.As the FCM method appeared,there was no apparent difference between the pure hydroxyapatite and1%,5%strontium substituted hydroxyapatite.Conclusion Strontium substituted hydroxyapatite has good biocompatibility,and10%,100%strontium substituted hydroxyapatite has weak cytotoxicity.