P-选择素在鼻息肉中的表达和机制研究

Expression of P-selectin in nasal polyps and its mechanisms

目的 探讨P 选择素 (P selectin)在鼻息肉中的表达及分析可能的机制。方法 分别采用免疫组化和逆转录聚合酶链反应 (reversetranscriptasepolymerasechainreaction ,RT PCR)检测了 2 4例慢性鼻窦炎患者鼻息肉中P 选择素的表达并以 9例正常鼻黏膜作为对照 ,同时采用酶联免疫吸附测定 (enzyme linkedimmunosorbentassay,ELISA)检测白细胞介素 5 (interleukin 5 ,IL 5 )对离体培养的血管内皮细胞表达P 选择素的诱导作用。结果 2 4例患者鼻息肉中P 选择素表达的阳性率为 79 1%(19/14) ,高于对照鼻黏膜的 2 2 2 % (2 /9) ,免疫组化显示P 选择素主要定位于血管周围和血管壁内皮细胞上 ,上皮细胞表层和上皮下间质中的炎性细胞也有部分表达。RT PCR及电泳结果显示鼻息肉组织中P 选择素 /β 肌动蛋白相对密度比值为 0 6 8± 0 11,高于对照组的 0 2 9± 0 0 8;IL 5诱导后培养血管内皮细胞上清液中P 选择素含量为 (5 3 4± 2 9)ng/L ,无诱导的内皮细胞上清液中P 选择素含量(2 1 3± 3 5 )ng/L。结论 鼻息肉中P 选择素的表达增高 ,并与浸润嗜酸粒细胞数量成正相关 ,经IL 5诱导的离体血管内皮细胞P 选择素合成也增强 ,表明P 选择素在鼻息肉的病理形成过程中具有一定的作用 ,并能被IL

Objective To investigate the expression of P-selectin and its mechanism in nasal polyps. Methods The expression of P-selectin was detected with immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in nasal polyps from 24 patients and nasal epithelium from 9 normals. The production of P-selectin in supernatants of isolated cultured vascular endothelial cells induced by interleukin-5 (IL-5) was detected with enzyme-linked immunosorbent assay (ELISA). Results The positive rate of P-selectin in nasal polyps was 79.1% (19/24) while it was 22.2%(2/9)in normals. The relative density ratio of P-selectin and β-actin were 0.68±0.11 in nasal polyps and 0.29±0.08 in normals indicated by RT-PCR and agrose electrophoresis. The concentration of P-selectin was (53.4±2.9) ng/L and (21.3±3.5) ng/L in the two groups respectively.Conclusions P-selectin upregulated in nasal polyps correlated with the infiltrated eosinophils and IL-5 could induce the expression of P-selectin protein in the isolated cultured endothelial cells. It suggested that upregulation of P-selectin may play a role in the pathogenesis of nasal polyps and can be enhanced by cytokine IL-5.