冠心病患者血浆低密度脂蛋白亚组份的研究

Study on plasa low density lipoprotein subfractions in patients with coronary heart disease

目的 :观察不同性别、不同年龄组冠心病患者低密度脂蛋白 (LDL)亚组份和血脂成分的变化 ,探讨这些变化与冠心病发病的相关性。方法 :用 2 %～ 16 %非变性聚丙烯酰胺梯度凝胶电泳法将LDL分为LDL1、LDL2 和LDL3 3个亚组分 ,染色后进行密度扫描测量主峰的泳动距离。用酶法测定血脂 ,用免疫比浊法测定载脂蛋白。结果 :男性冠心病患者LDL3 (小密LDL)较女性患者增多 ,随着年龄的增加 ,LDL3 颗粒也增多。冠心病患者与对照组比较TC、TG、LDL C、apoB10 0 明显增高 ,P <0 .0 1,HDL C和apoAⅠ明显降低 (P <0 .0 5 )。结论 :LDL3 亚组分的增高与冠心病发病密切相关。

Objective:To probe the relationship between the LDL subfractions and the development of CHD.Methods:Three subfractions of LDL(LDL 1, LDL 2 and LDL 3) were separated by 2%～16% non denaturing polyacrylamide gel electrophoresis in CHD patients of different sexes and ages. The bands were scanned after staining to measure the electrophoresis distance,blood lipids were measured by enzyme methods,and apolipoproteins A I and B 100 were determined with immunosedimentation.Results:The LDL 3 (small dense LDL) of male CHD patients was higher than that of the female and increased with age.The TC,TG,LDL C and apoB 100 concentrations significantly increased compared with those of the control group(P<0.01). The HDL C, apo AI reduced significantly(P<0.05).Conclusion:The low density lipoprotein subfraction 3 is closely related to the development of coronary heart disease.