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整合素a_5亚单位在大鼠角膜碱烧伤愈合中的表达 被引量：1

<title>Expression of integrin α_5 subunit during corneal alkaline burn wound healing in rats
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摘要 目的　观察碱烧伤后角膜创面上皮愈合过程中整合素 α5亚单位的表达 ,探讨整合素 α5在角膜上皮损伤愈合中的作用。方法　 Wistar大鼠 1 8只 ,随机分为 6组 ,每组 3只 ,其中 1眼为碱烧伤模型 ,另 1眼为自身对照眼。对制造模型眼后 0、7h、1、3、7、1 4d的角膜标本 ,HE染色行组织病理学检查、间接免疫荧光和免疫组织化学染色观察整合素 α5亚单位的表达与分布。结果　伤后 7h,邻近上皮细胞开始表达 α5亚单位 ,伤后 1、3、7d,角膜上皮层显示 α5表达逐渐增加 ,差别有显著性 (P<0 .0 5)。伤后 1 4d,实验组表达仍呈上升趋势。结论　整合素 <Abstrcat>ObjectiveTo observe the expression of integrin α5 subunit during corneal alkaline burn wound healing in rats. MethodsEighteen Wistar white rats(body weight 200～250g) were divided into six groups. Three rats in each group were made into corneal alkaline burn model by 0.5mol·L-1 sodium hydroxide solution. The cornea were surgically removed at six different time points(0?7h,1?3?7?14d)after operation, three rats for each time. The localization and expression of integrin α5 subunit was determined by indirect immunofluorescence and immunochemical methods. ResultsThe expression of integrin α5 subunit of experimental groups increased in alkaline burn cornea at seventh hour after operation, and it increased gradually after 1day,3,7,14days.ConclusionThe expression of integrin α5 increased markedly gradually during corneal alkaline burn wound healing, as promoted the adhesion of cellcell and cellECM.
作者 张妍霞 王传富 张世杰
机构地区 复旦大学附属眼耳鼻喉科医院眼科 青岛大学医学院附属医院眼科
出处 《眼科新进展》 CAS 2002年第5期305-308,共4页 Recent Advances in Ophthalmology
基金 .NULL.
关键词 角膜 碱烧伤 整合素 大鼠 cornea alkaline burn integrin rat
分类号 R779.13 [医药卫生—眼科]
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参考文献14

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同被引文献13

  • 1Schaumberg DA,Dana MR,Christen WG,Glynn RJ.A systematic overview of the incidence of posterior capsule opacification[J].Ophthalmology 1998; 105:1213-1221.
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  • 7Yoshino M,Kurosaka D,Obazawa M,Takayama F.Presence of alpha5 betal integrin and fibronection in the anterior subcapsular cataract[J].Nippon Ganka Gakkai Zasshi 2001; 105:83-87.
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  • 10Yee KO,Ikari Y,Bodary S,Schwartz SM.Kistrin inhibits human smooth muscle cell inter-action with fibrin[J].Throm b Res 2000; 97:39-50.

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眼科新进展
2002年 第5期

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