己酸菌筛选新途径及工艺参数正交试验

New Approaches to Caproic acid-producing Bacteria Screening&Orthogonal Tests of Technical Parameters

采用加大琼脂用量制成固体斜面培养基,以稀释分离、真空培养的方法进行己酸菌分离纯化,并采用正交试验法对己酸菌的生长繁殖条件进行了探讨,其影响因素依次为酒度、pH值、培养时间、培养温度及热处理温度,优化因子为pH6,酒度2%,热处理温度80℃,培养温度30℃,培养时间6d。

The solid slant culture medium,prepared by increasing the use level of agar;the separation and purification of caproic acid-producing bacteria,processed by the techniques of separation by dilution and vacuum culture;then the growth and proliferation conditions of caproic acid-producing bacteria were investigated by orthogonal tests.The results indicated that the sequence of the influ-encing factors were alcohol content ,pH values,culture time ,culture temperature,and thermal treatment temperature.Generally,the pH value of optimized genes was6,alcohol content was2%,thermal treatment temperature at80℃,culture temperature at30℃,and culture time was6days.(Tran.by YUE Yang)