摘要
目的 研究抗线粒体抗体 (AMA)M2 亚型检测对原发性胆汁性肝硬化 (PBC)的诊断价值。方法 应用酶联免疫吸附试验 (ELISA) ,检测人血清中M2 抗体含量。共检测正常人 2 0名 ,PBC患者 3 7例 ,其他肝病患者 5 0例。结果 ELISA法检测M2 抗体重复性好 ,灵敏度高 ,特异性强。 3 7例PBC患者中有 3 6例阳性 (>5U/ml) ,只有 1例阴性。而其它肝胆系患者 5 0例 ,3例为假阳性。灵敏度为 97.3 % ,特异性为 94.0 %。正常人均为阴性。PBC患者M2 抗体滴度为 (73 .66± 5 5 .93 )U/ml,较正常人 (1.60± 1.13 )U /ml显著升高 (P <0 .0 1) ,其他肝胆系患者M2 抗体滴度 (2 .90±3 .16)U/ml与正常人相比无显著差别 (P >0 .0 5 )。结论 M2
Objective Study on the diagnostic value of anti mitochondrial antibody type 2(M 2 antibodies) in primary biliary cirrhosis (PBC). Methods ELISA method was used for the detection of M 2 antibodies. 20 healthy volunteers, 37 PBC patients and another 50 patients with other hepato biliary diseases were investigated.Results The positive rates of M 2 antibodies were 97.3% in PBC patients with high titers (73.66±55.93U/ml),6.0% in other hepato biliary disease patients with low titer (2.90±3.16U/ml), and all negative in normal controls (1.60±1.13U/ml).Conclusion M 2 antibody detection is an important sero immunological marker for the diagnosis of PBC.
出处
《肝脏》
2002年第3期153-155,共3页
Chinese Hepatology