摘要
目的鉴定河北中药材市场柴胡的真伪。方法对从河北中药材市场采购的28份柴胡药材的ITS2序列进行PCR扩增,同时从Gen Bank下载7个样本序列。采用Codon Code Aligner软件进行序列拼接,采用软件MEGA 6.0分析比对,计算其种间、种内的K2P距离以及各序列的变异位点并进行聚类分析。结果 28份样品中有21份为柴胡,3份为黑柴胡,4份为伪品。结论 ITS2序列能够作为柴胡药材鉴别的有效方法之一,对药材市场的规范和临床用药安全具有重要意义。
Objective To identify the authenticity of Bupleuri Radix in the market of Hebei province.Methods In this research,the ITS2 sequence of 28 samples of Bupleuri Radix that were bought from the market in Hebei province was amplified by PCR,and the sequence of seven samples was downloaded from Gen Bank.Aligner Codon Code software was used for sequence alignment,the MEGA6.0 analysis was used to calculate the K2 P distance,and the mutation sites of each sequence was analyzed.Results Among the 28 Bupleuri Radix samples,there were 21 samples for Bupleurum chinese,3 for B.smithii,and 4 for the false.Conclusion ITS2 sequence can be used as an effective method to identify the authenticity of Bupleuri Radix,it is great value to the medicine market standard and the clinical medication safety.
出处
《中草药》
CAS
CSCD
北大核心
2016年第9期1583-1588,共6页
Chinese Traditional and Herbal Drugs
基金
河北省中医药管理局课题(2015081)
河北中医学院博士科研启动基金(BSZ2015003)
河北中医学院中药学重点学科
河北省教育厅青年基金(QN2015073)
河北省现代农业技术体系中药材创新团队项目(7000120081)
关键词
柴胡
ITS2序列
药材
鉴别
条形码
Bupleuri Radix
ITS2 sequence
Chinese medicinal materials
identification
barcoding
