鼻咽癌克隆株及其高淋巴道转移裸鼠移植瘤的细胞遗传学改变

CYTOGENETIC CHANGES ON NASOPHARYNGEAL CARCINOMA SUBCLONE CNE 2Z H5 AND ITS TRANSPLANTED TUMOR WITH HIGH LYMPHATIC METASTASIS IN NUDE MICE

目的 研究鼻咽癌克隆株CNE2ZH5及其高淋巴道转移裸鼠移植瘤离体培养细胞的细胞遗传学改变及其与肿瘤生物学特性之间的关系。 方法 染色体G显带后按人类细胞遗传学国际命名体制(ISCN)进行核型分析。 结果 CNE2ZH5的染色体数目在83～93之间,众数为86～88;高淋巴道转移移植瘤的染色体数目在64～103之间,众数为80～83。两种细胞的染色体数目均呈非整倍性变化,为亚四倍体,并有众多恒定或经常出现的标记染色体,其带型复杂难以辩认,为多次结构畸变的产物。 结论 与CNE2ZH5相比较,移植瘤的染色体数目畸变范围较广,众数也有所减少。

PURPOSE To study the cytogenetic changes of nasopharyngeal carcinoma (NPC) subclone CNE 2Z H5 and its transplanted tumor with high lymphatic metastasis in nude mice and the relationship between these changes and the biological behaviors of NPC.METHODS G banding of chromosomes and then karyotype were analysized with an international system for human cytogenetic nomenclature (ISCN).RESULTS The chromosome number of CNE 2Z H5 cells varied from 83 to 93 and the modal number was 86～88; the chromosome number of transplanted tumor cells from 64 to 103 and the modal number 80～83. Both kinds of cells were hypotetraploid and contained many consistently or frequently found marker chromosomes whose banding patterns were too complicated to be recognized as a result of several structural aberrations.CONCLUSION Compared with CNE 2Z H5,the transplanted tumor has a wider range of chromosome number abnormalities and a smaller modal number,suggesting that the difference may correlate with its behavior of high lymphatic metastasis.