稀释液中添加海藻糖对猪冷冻精液质量参数和活性氧的影响

Influence of Trehalose Supplemented in Diluent on Quality Parameters and Reactive Oxygen Species Levels of Frozen Boar Spermatozoa

本试验旨在探讨海藻糖对猪精子冷冻效果以及精子内活性氧(ROS)的含量影响。试验分对照组和4个海藻糖处理组(0.025,0.05,0.1和0.2 mol/L)。精子冷却后,添加0.1 mol/L海藻糖处理组精子活力显著(P<0.05)高于对照组,而精子内和稀释液中各组间ROS发生量都没有显著的差异。精子冷冻解冻后,添加0.05 mol/L海藻糖处理组的精子活力显著(P<0.05)高于对照组;添加0.05 mol/L和0.1 mol/L海藻糖处理组的精子生存率显著(P<0.05)高于对照组;添加0.1 mol/L海藻糖处理组顶体完整的精子百分比显著(P<0.05)高于对照组;4个海藻糖处理组膨胀精子百分比都显著(P<0.05)高于对照组;添加0.025 mol/L海藻糖处理组ROS水平显著(P<0.05)低于对照组。结果表明:海藻糖对精子冷冻保存是有益的,且能抑制精子内ROS的发生,但它的作用机制有待于进一步的研究。

The objective of this study was to investigate the effect of trehalose on freezing efficiency of boar sperm and level of reactive oxygen species(ROS) in spermatozoa.Experiments were divided into control and four trehalose treatment groups(0.025,0.05,0.1 and 0.2 mol/L).The percentage of mobile sperm in 0.1 mol/L trehalose group was significantly higher(P<0.05) than that of control after cooling.The percentage of mobile sperm in 0.05 mol/L trehalose group,viable sperm in 0.05 and 0.1 mol/L trehalose group,intact sperm in(0.1 mol/L) trehalose group and swollen sperm in 4 trehalose groups were significantly higher(P<0.05) than those of control after freeze-thawing,respectively.Level of ROS in 0.025 mol/L trehalose group was significantly lower(P<0.05) than that of control after freeze-thawing.These results showed that supplementation of trehalose had a beneficial effect on cryopreservation of spermatozoa,and could prevent ROS generating from spermatoza.