蜜蜂大肚病致病菌的分离与鉴定

Isolation and Identification on “DistendingAbdomen” of Pathogenic Bacteria from Honeybee

从患大肚病的蜜蜂中分离出致病菌,进行形态观察、回归实验、生理生化指标鉴定和药敏实验等研究。发现该菌为G-球杆菌,不溶血,在普通肉汤培养基上呈乳白色圆形菌落,在SS培养基上不生长,接触酶为阳性,不产生H2S,不能还原硝酸盐。药敏纸片实验对菌必治、环丙沙星等高度敏感,庆大霉素、硫酸阿米卡星的半数抑菌量(IC50)分别为8.73iu/ml和36.92iu/ml,用20-30iu/ml剂量的庆大霉素预防,可有效地抑制大肚病的发生和蔓延。通过对照和鉴定,再经血清型确定,蜜蜂大肚病中分离出的致病菌为黄杆菌。

The pathogenic bacteria of 'distending abdomen' has been isolated from honeybee,Studies including morphologic observation,regression test,physiological and biochemical index appraisal and drug sensitivity test.The results showed that the pathogenic bacteria is gram-negative ball coli,no hemolysis,there are milk-white circular colonies on ordinary broth culture medium,it does not grow in SS medium,catalase shows positive,do not produce H 2 S,and it is negative in nitrate reduction test.Medicine sensitive piece of paper experiments are highly sensitive for rocephin and ciprofloxacin,the IC 50 of gentamycin and amikacin are 8.73iu/mland36.92iu/ml,gentamycin (20-30iu/ml) can prevent effectively occurrence and spread of 'the bigbelly disease'.Through the comparison and Identification,then the blood serums sure,the pathogenic bacteria isolated is flavobacterium from honeybee of 'distending abdomen'.