华南地区鼻咽癌13号和14号染色体长臂发生高频率等位基因杂合性缺失(英文)

High frequency loss of heterozygosity on the long arms of chromosomes 13 and 14 in nasopharyngeal carcinoma in Southern China

目的 分析鼻咽癌 (NPC) 13号染色体长臂 (13q)和 14号染色体长臂 (14q)上 2 1个位点的等位基因杂合子丢失(LOH) ,并分析这些位点的LOH与NPC临床病理及EBV感染的关系。方法 用聚合酶链反应 (PCR)为基础的微卫星多态性分析技术结合基因扫描和基因绘图技术对 6 0例NPC进行LOH分析。结果 13q染色体发生一个或多个位点LOH频率为 78% ,高频率LOH(大于 30 % )位点集中于 13q12 3 q14 3和13q32附近。 14q染色体发生至少一个位点LOH的频率为 80 % ,高频率丢失位点集中于 14q11 q13、14q2 1 q2 4和14q32附近。 13q31 q32位点的LOH与低滴度血清EBVEA/IgA有关 ;14q染色体的LOH与NPC细胞的分化差有关。结论 华南地区鼻咽癌在 13q和 14q染色体发生高频率的LOH ,这些缺失区可能存在多个在NPC发生发展过程中起重要作用的肿瘤抑制基因。

To investigate the loss of heterozygosity (LOH) on chromosomal arms 13q and 14q in nasopharyngeal carcinoma (NPC) using 21 microsatellite polymorphic markers an d to study whether there is a correlation between LOH and clinicopathologic para meters and/or Epstein Barr virus (EBV) infection in NPC Methods Sixty cases of NPC were studied using polymerase chain reaction based microsa tellite analysis with genescan and genotyping techniques Results LOH was detected on 13q in 78% of NPC tumors, high frequency LOH loci (more than 30%) clustered to 13q12 3 q14 3 and 13q32 On chromosome 14q, LOH was detec ted in 80% of NPC tumors; high frequency LOH loci clustered to 14q11 q13, 14q21 q24 and 14q32 High frequency LOH at 13q31 q32 correlated with a lower l evel of EBV infection; LOH on chromosome 14q was closely associated with poor di fferentiation of NPC tumor cells Conclusion Our results suggest that in NPC, LOH on chromosome 13q and 14q are common geneti c events, and putative tumor suppressor genes (TSG) residing in these regions ma y be involved in tumorigenesis