Diagnostic value of human cytomegalovirus late mRNA detection in active intrauterine infection

检测人巨细胞病毒晚期mRNA在诊断宫内活动性感染的价值(英文)

To study the diagnostic value of human cytomegalovirus (HCMV) late mRNA detection in active intrauterine infection Methods The HCMV late mRNA in peripheral blood of 42 HCMV IgM positive pregnant women and their fetal attachments (such as chorionic villi, amniotic fluid, umbilical blood and placenta) were detected by reverse transcription polymerase chain reaction (RT PCR) Results Late mRNA was detected in 23 of 42 HCMV IgM positive cases, a rate of 54 3% Fetal appendages in 13 cases of late mRNA positive mothers were also tested, of which 7 were positive, with a vertical transmission rate of 53 8% In 12 late mRNA negative mothers, only 1 case of fetal appendages tested was positive, with a vertical transmission rate of 8 3% There was significant difference between the transmission rates of these two groups Conclusions Positive results of HCMV IgM cannot accurately reflect the activity of HCMV at the time of testing However, the activity of HCMV is closely related to the mother fetus vertical transmission rate As an indicator of active HCMV infection, late mRNA can not only reflect the mother fetus transmission rate during active HCMV infection, but also provide some information about HCMV activity in fetal tissue

探讨人巨细胞病毒晚期mRNA检测在宫内活动性感染中的应用价值。方法 采用逆转录 PCR法对 42例HCMV IgM阳性孕妇外周血及部分胎儿附属物进行HCMV晚期mRNA检测。结果 42例IgM阳性孕妇检出晚期mRNA 2 3例 ,两者符合率为 54 7%。对其中 1 3例mRNA阳性孕妇胎儿附属物进行检测发现 7例阳性 ,母婴传播率为 53 8% ;而 1 2例mRNA阴性HCMV DNA阳性孕妇胎儿附属物中仅 1例阳性 ,母婴传播率为 8 3 % ;两者有非常显著性差异。结论 HCMV IgM的阳性结果并不能完全准确地反映受检时HCMV的活动状态。HCMV的活动状态与母婴传播率密切相关 ,mRNA的检测不仅能够正确反映HCMV活动性感染时的宫内传播率 ,而且可以了解受检时胚胎或胎儿组织内HCMV的活动状态 ,并估计其愈后。