摘要
To examine the effects of procaine and lidocaine on intracellular Ca 2+ release from sarcoplasmic reticulum ryanodine sensitive Ca 2+ stores Methods The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils Levels of intracellular Ca 2+ concentration in the slices were measured by microfluorometry The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds Then, the medium was switched to physiological medium After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min Following incubation, the slice was superfused with physiological medium until the end of the experiment The effects of procaine and lidocanin (100 μmol/L) on caffeine evoked Ca 2+ release were evaluated by adding them to the medium after high K + medium perfusion Results Caffeine induced a marked increase in intracellular Ca 2+ concentration which was then decreased 12% upon the addition of procaine ( P 【0 05); however, lidocaine, did not induce a similar inhibitory reaction Conclusion Procaine inhibits ryanodine receptor mediated Ca 2+ release from intracellular Ca 2+ stores, while lidocaine may inhibit Ca 2+ release through other mechanisms
目的 观察普鲁卡因和利多卡因对细胞内织网内ryanodine敏感的钙离子释放的影响。方法 采用 60 -80g的蒙古鼠海马切片 ,用显微荧光法测定细胞内钙离子浓度。将切片用含 50mmol/LKCl的人工脑脊液灌注 3 0秒 ,然后用人工脑脊液灌注 ,5分钟后用含 2 0mmol/L咖啡因的脑脊液灌注 2分钟 ,然后换为人工脑脊液直到实验结束。将 1 0 0umol/L的普鲁卡因或利多卡因分别加入到高钾后的灌注液中观察它们对钙离子释放的影响。结果 咖啡因可致细胞内钙离子浓度的明显增高 ,而普鲁卡因可使这种增高减少 1 2 % ,而利多卡因则无此抑制作用。结论 普鲁卡因可抑制ryanodine受体介导的细胞内钙离子释放 。
