Experimental study on a novel compound extracted from Traditional Chinese Medicine for treatment of alveolar echinococcosis

一种新的TCM提取化合物治疗泡型包虫病(泡球蚴病)的实验研究(英文)

To evaluate the efficacy of a novel compound derived from Traditional Chinese Medicine (TCM) for treatment of alveolar echinococcosis in the Kuming mouse Methods The compound was extracted from TCM by serial procedures including amalgamation of TCM drugs, boiling in a beaker, cold condensation using a reflux instrument, evaporation by mild heating and finally, formation of a deep brown powder An animal model of alveolar echinococcosis was established concurrently A bit of alveococcus tissue derived from alveolar echinococcosis in the mouse was transplanted into the peritoneal cavity of the musculus of a Kuming mouse The duration of infection after inoculation was classified as either 1 week or 10 weeks; the animals were again divided into treated groups and control groups Estimation of the alveococcus inhibitory rate and electron microscopic observation served as the main methods for assessment of efficacy The infected mice in the treated groups were intra gastrically administered once per day with the TCM extractive powder at doses of 20 mg·kg 1 for three months continuously All the animals were sacrificed and examined half a month after the end of TCM treatment Results Total wet weight of alveococcus collected from the 9 mice in the treated group after one week of infection was 37 8 g ( ±s , 4 196±2 090 g), which was markedly lower than the amount collected from 10 mice of the control group (121 3 g, ±s , 12 1±4 3 g) Therefore, the inhibitory rate of the alveococcus was 65 7% ( P 【0 01 ) Similarly, the total wet weight of alveococcus from 7 mice of the treated group after 10 weeks of infection was 4 3 g ( ±s , 0 62±1 21 g), which was much lower than the amount collected from 6 mice of the control group (17 85 g, ±s , 2 93±3 28 g) The inhibitory rate of alveococcus was 80 6% ( P 【0 01) Furthermore, the ultrastructural appearances of alveococcus showed obvious differences between the two groups Conclusion TCM extractive powder for treatment of murine alveococcosis is considered to be a hopeful anti echinococcus compound it is a topic for further study

目的 评估一种新的TCM提取化合物治疗小鼠泡型包虫病的效果。方法 首先从TCM中提取一种化合物 ,要通过一系列程序包括中药混合 ,置平底烧瓶内加热煮沸 ,用冷凝回流装置和微火蒸发 ,最后形成为深棕色粉剂。同时建立泡球蚴病动物模型 ,从感染泡球蚴病的小鼠获取小片泡球蚴组织 ,移植接种 50只昆明小鼠腹腔内 ,接种后受染期分为 1周感染和 1 0周感染 ,二者再分为治疗组和对照组。泡球蚴抑制率测定和电镜检查作为判定疗效的主要方法。治疗组感染鼠每天灌胃TCM提取化合物一次 ,剂量 2 0mg/kg ,连用 3个月。TCM治毕半月 ,处死剖检所有动物。结果 1周感染后治疗组 9只鼠的泡球蚴总湿重 3 7 76g( x±s,4 1 96± 2 0 90g) ,明显低于 1 0只对照鼠 ( 1 2 1 2 94g , x±s,1 2 1 2 9± 4 3 0 5g)。泡球蚴抑制率为 65 7% (P <0 0 1 )。同样地 ,1 0周感染后治疗组 7只鼠的泡球蚴总湿重 4 3 2 6g( x±s,0 61 9± 1 2 0 7g) ,明显低于 6只对照鼠 ( 1 7 857g , x±s,2 92 6± 3 2 75g)。泡球蚴抑制率为80 6% (P <0 0 1 )。泡球蚴超微结构在治疗组与对照组之间显示明显的区别。结论 TCM提取粉治疗小鼠泡球蚴病 ,是一种有希望的抗包虫化合物 ,今后应继续进行研究。