6种木材白腐菌对山杨材木质素分解能力的研究

STUDY ON THE DEGRADING ABILITY TO DAVID POPLAR LIGNIN OF SIX SPECIES OF WOOD WHITE-ROT FUNGI

由于不同的木材腐朽菌的生理特性不同 ,所分泌的酶及酶的活性各不相同 ,因此 ,不同的腐朽菌分解木材的各种成分及相对速度就各不相同 ,而且对于木质纤维基质会有不同的中间代谢产物。本项研究选择了火木层孔菌 (Phelliusigniarius)及另外 5种木材分解能力较强的阔叶树上的白腐菌 :粗毛盖菌 (Funaliagallica)、三色革裥菌 (Lenzitestricolor)、冬拟多孔菌 (Polyporellusbrumalis)、偏肿拟栓菌 (Pseudotrametesgibbosa)和血红密孔菌 (Pyc noporussanguineus) ,研究了它们对山杨木材木质素的分解能力 ,测定了经 6种白腐菌分解一定时期的山杨木材木质素的含量 ,作为木材白腐菌对山杨木材木质素生物降解机制的初步研究 ,旨在为山杨木材生物制浆造纸提供应用基础理论研究 ,同时也可为木质素合理的生物转化为有用的化学品、生物漂白、酶处理防止机械浆的返黄、废水治理、纤维素酶解糖化的微生物前处理等提供相关的借鉴研究 ,以期在生产实践中减轻环境污染并充分利用木质素资源。在无菌的条件下 ,将山杨木片样品分别放入以上 6种白腐菌的平板培养基中受菌侵染 ,一定时间后取出 ,去除木片表面的菌丝体 ,然后分别测定未腐朽材和受菌侵染 4 0d、6 0d、80d和 12 0d时木片样品中木质素的含量 ,分析

Because different wood rot fungi have different physiological properties, and they can produce different enzymes with different activity, different wood rot fungi degrade different compositions of wood with different velocities, and the middle materials degraded by different wood rot fungi are different. In order to offer a applicating basic theory study to biopulping with david poplar wood, and offer a related study to microbial conversions of lignin to useful chemicals, biobleaching, enzymes treatment preventing from becoming yellow of mechanical pulping, wasted water control, microbial pretreatment of cellulose sugaring by enzymes, and decrease environment pollution in paper industry and make full use of lignin resources, this study selected Phellius igniarius and other 5 wood rot fungi with higher wood degrading ability living on broad leaf trees, i.e. Funalia gallica, Lenzites tricolor, Polyporellus brumalis, Pseudotrametes gibbosa, Pycnoporus sanguineus, tested their degrading ability to david poplar wood lignin, measured the lignin content change of david poplar wood after a period of time degraded by 6 wood rot fungi, which is the first study on the biodegradation mechanism of lignin by wood white rot fungi. Under sterile conditions, the standard david poplar wood samples were put plate media of 6 species white rot fungi and degraded by them. After 40 d, 60 d, 80 d and 120 d, the rotted wood samples were taken out respectively and the mycelium on the wood samples were brushed off. Then the lignin contents of primitive wood sample and rotted wood samples were measured. Results showed the lignin degrading ability of 6 species of wood white rot fungi is different, it is Pycnoporus sanguineus, Pseudotrametes gibbbosa, Polyporellus brumalis, Lenzites tricolor, Funalia gallica and Phellius igniarius in turn. If considering the degraded extent of cellulose, Polyporellus brumalis is the better wood white rot fungi with higher ability of degrading lignin and lower ability of degrading cellulose.