摘要
采用热休克的方法阻止第二级体排出诱导鲫鱼异源三倍体（Allotriplogidy）。结果表明，诱导鲫鱼异源三倍体较为适宜的条件是，授精后的4-6分钟，采用38℃．1．5分钟或37℃，2分钟的热休克处理，均可导致100％的三倍化率，出苗率最高为对照组的75．8％，本文还对鲫鱼三倍体诱导技术及影响这一技术的环境因素（特别是地理区域、受精水温等对诱导成功率的影响）以及三倍体生长和利用等问题进行了分析和讨论。
Allotriploidy were producted by heat shock in crucian carp. For the induction of allotriploidy, Eggs of crucian carp were fertilizated with sperm of carp and arrested the second meitotic division. Experimental result showed that the most effective methods of inducing allotriploidy were heat shock of 37℃ or 38℃ applied at 4-6ruin postfertilization for duration of 1. 5 or 2min. these treatments resulted in triploidy inductio
出处
《内蒙古民族大学学报》
1998年第1期20-29,19,共11页
Journal of Inner Mongolia University for the Nationalities
基金
内蒙古教育厅资助
关键词
鲫鱼
三倍体
热休克
染色体组工程
Crucian carp
Triploidy
Heat shock
Genome engineering
