14个品种冬小麦过氧化物酶(POD)同工酶研究

Stady on Peroxidase (POD) Isoenzymes of 14 Varieties of Winter Wheats

采用聚丙烯酰胺凝胶垂直板电泳技术对１４ 个品种冬小麦过氧化物酶（ＰＯＤ） 同工酶进行了研究，发现各品种冬小麦的ＰＯＤ 同工酶谱可明显分成Ａ、Ｂ 两个区。其中１３ 个品种Ａ 区有４ 条带，一个品种Ａ 区是６ 条带。Ｒｆ 分别为０－０５ 和０－１９ 的２ 条一级酶带是所有供试品种所共有的。Ｂ 区酶带少，且多为扩散带。Ｒｆ 值为０－２６ 的酶带与产量及农艺性状有关，有此酶带的品种返青率和理论产量显著高于无此带的品种；

POD isoenzymes of 14 varieties of winter wheats were isolated by PAGE. It was found that the zymogram of POD isoenzymes could be divided region A and region B. In region A, 13 varieties of winter wheats had 4 bands and one had 6 bands. There were two bands(Rf were 0.05 and 0.19 vespectivdly)which appeared in all varieties of winter wheats. In region B, there were a few bands,most of them were diffuse bands. The band (Rf was 0.26) related to the ratio of turning green and theorefical yield. The ratio of turning green and theoretial yield of the varieties with this band were higher than that of those without this band. The stem height and spike length of varieties with this band were higher than that of those without this band pronuncedly.