生理盐水提取柞蚕蛹蛋白及SDS-PAGE分析

Normal saline extraction of oak silkworm protein and SDS-PAGE analysis

以柞蚕蛹为原料,分别采用去离子水、生理盐水、30%的乙醇浸泡,匀浆,等电点沉淀提取粗蛹蛋白,凯式定氮法测定蛋白含量,确定一种新的提取工艺。石油醚脱脂得蛋白粉,采用SDS-PAGE凝胶电泳进行分析。结果表明:粗蛋白得率分别为22.31%、38.98%、10.79%;粗蛋白中蛋白质含量分别为10.44%、55.00%、47.86%,因此确定使用生理盐水提取蛹蛋白,经脱脂后蛋白含量70.0%;脱脂蛋白为6条带,178kDa、64kDa和62kDa、42kDa、30kDa、28kDa、16kDa。

In this paper,oak silkworm protein was extracted by soaking in deionized water,normal saline and 30% ethanol,homogenate and isoelectric precipitation with oak silkworm as raw material.Then nitrogen was analyzed by Kjeldahl method,a new extract technology was developed.Protein power was defated with petroleum ether and analyzed by SDS-PAGE.Results showed that : crude protein extraction rate were 22.31%,38.98% and 10.79% respectively.Protein content in crude protein were 10.44%,55.00% and 47.86% respectively.So normal saline was selected as solvent,protein content arrived 70.00% after defatting,6 bands were : 178kDa,64kDa,62kDa,42kDa,30kDa,22kDa and 16kDa.