改进的DPPH·法测定葡萄籽原花青素抗氧化能力研究

Study on antioxidant efficiency of grape seed proanthocyanidin by the modified DPPH· method

采用改进的DPPH.法,引用一个新参数—自由基清除能力AE(Antiradical efficiency)来测定葡萄籽原花青素抗氧化、清除自由基能力。它包含DPPH.原始质量浓度减少至50%(稳定态)时葡萄籽原花青素的添加量(EC50)及达到该状态的时间(TEC50)2个因素。结果表明:在梯度洗脱中,随着乙醇浓度的提高,产物抗氧化、清除自由基的能力也相应地提高。GSP(葡萄籽原花青素粗提物)、GSP1(纯化物1)、GSP2(纯化物2)、GSP3(纯化物3)、GSP4(纯化物4)、VC、芦丁的AE分别为5.6×10-4、6.6×10-4、7.9×10-4、9.8×10-4、11.5×10-4、68.6×10-4、1.4×10-4。

The efficiency of antioxidant and antiradical of the proanthocyanidin extracted from grape seed was determined by modified DPPH· assay.It depended on the introduction of a new parameter-antiradical efficiency(AE),which included two factors 50% the initial DPPH· concentration(EC50) and the time to reach the steady state to EC50 concentration(TEC50).The result showed that the efficiency increased with the purified degree of the proanthocyanidin from grape seed during the gradient elution.The antiradical efficiency(AE) of the GSP(proanthocyanidinext extracted from grape seed),GSP1,GSP2,GSP3,GSP4,VC and rutin were 5.6×10-4,6.6×10-4,7.9×10-4,9.8×10-4,11.5×10-4,68.6×10-4,1.4×10-4 respectively.