摘要
以绿色木霉HY-07为发酵菌株,通过单因素和正交试验对纤维素酶的液体发酵条件进行了优化。最佳产酶条件为:250mL三角瓶添加Mandels无机营养液75mL,添加玉米芯2.0%,蛋白胨0.05%,吐温-80 0.1%,pH值为5.0;菌龄78h,接种量4.0%,转速150r/min、28℃培养102h。其酶活为401.7U/mL,比优化前提高了44.6%。
Through single factor and orthogonal tests,the technological optimization of cellulase production using liquid-state fermentation was studied by trichoderma viride HY-07.The optimal conditions of enzyme production were as following:75mL Mandels inorganic nutrition liquid in 250mL flask, corn cob 2.0%,peptone 0.05%,Tween 80 0.1%,pH value 5.0,seed age 78h,inoculum volume 4.0%,revolving speed 150rpm/min 30℃for 102h.The enzyme activity of cellulase reached 401.7U / (U/mL) under the conditions mentioned above,the enzyme activity was increased by 44.6% contrasted to the initial.
出处
《中国酿造》
CAS
北大核心
2008年第12X期35-37,共3页
China Brewing
基金
陕西省科技攻关项目(2007JK01-13-2)
咸阳市科技攻关项目资助(K0311-2)
陕西科技大学自然科学基金项目资助(ZX06-17)
关键词
纤维素酶
绿色木霉
液体发酵
优化
cellulose
trichoderma viride
liquid state fermentation
optimization
