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柔嫩艾美耳球虫(广东株)子孢子折光体抗原p28基因的分析

Analysis of a Refractile Body Antigen p28 Gene of Eimeria tenella(Guang Dong Strain) Sporozoites
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摘要 将克隆的柔嫩艾美耳球虫广东株p28基因与柔嫩艾美耳球虫MerckstrainLS18株的折光体抗原p28基因和USDA-APIno.24株折光体抗原GX3262基因进行了序列比较.发现广东株和MerckstrainLS18株的折光体抗原核苷酸序列和氨基酸序列的同源性分别为99.9%和100%,且基因内富含AGC重复顺序;而GX3262的基因序列为P28基因C-端的组成部分,并无特别的疏水和亲水区. A cloned refractile body antigen p28 gene of Eimeria tenella (Guangdong Strain) sporozoites was compared with another one from Merck strain LS18 and a DNA fragment named GX3262. Sequence analysis revealed that the cloned p28 gene had a high homology with Merck strain LS18’s and showed 99.9% and 100% homology at nucleotide and amino acid level respectively. At p28’s carboxy terminus was the sequence of another partially protective, recombinant antigen, GX3262.
出处 《中山大学学报论丛》 1996年第S1期164-169,共6页 Supplement to the Journal of Sun Yatsen University
关键词 柔嫩艾美耳球虫 p28基因 序列分析 Eimeria tenella p28 gene sequence analysis

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