摘要
ＥＭＳ诱变的细胞经过Ｂｒｄｕ递增浓度的定向筛选，建立了可以抗３００ｍｇ／Ｌ浓度Ｂｒｄｕ的斜纹夜蛾胸苷激酶缺陷型细胞株．经［３Ｈ］胸苷磷酸化反应测定胸苷激酶（ＴＫ）活力表明。
A Spodoptera litura cell line deficient in thymidine kinase activity (Sl TK -) was isolated by mutagenesis with Ethylmethanesulfonate (EMS) and serial passages in the presence of 5’ bromodeoxyuridine(Brdu) at stepwise increasing concentrations. The resulted Sl TK cell line was confirmed to be lacking in TK activity by unable to catalyze the phosphorylization of thymidine.
出处
《中山大学学报论丛》
1996年第2期35-38,共4页
Supplement to the Journal of Sun Yatsen University
关键词
斜纹夜蛾
胸苷激酶
细胞
Spodoptera litura
thymidine kinase activity
cell
