属间远缘杂交水稻耐铁毒性研究

Study on Tolerance to Iron Toxicity of Intergeneric Hybrid Rice ( Oryza sativa L .)

以吉田昌—培养液为对照，添加不同浓度ＦｅＳＯ４作为铁毒胁迫处理，对二个属间远缘杂交水稻“远诱１号”和“遗传工程水稻１号”及三系杂交组合“汕优６３”二叶龄幼苗进行耐铁毒性比较鉴定．试验结果表明，３个试验材料的耐铁毒性以高粱稻远诱１号及三系稻汕优６３较强，遗传工程水稻１号耐铁毒性相对较弱．根据溶液中Ｆｅ２＋／Ｆｅ３＋的变化规律，稻体内持铁量的差异，稻株根系氧化力的区别，拒斥可能是汕优６３的耐铁毒性生理基础，遗传工程水稻１号的耐性机制为容耐，而远诱１号的耐铁生理基础兼具拒斥与氧化双重机制而偏倚于主动氧化机制．体内钾、硅、镁、钙含量与铁离子含量密切相关，丰富的钾、硅。

Tolerance to excess ferrous iron ion (Fe 2+ ) stress of two intergeneric hybrids of rice crossed Sorghum (YR) and maize (GR) are comparatively studied with comparison of Shanyou 63 (SR 1,F 1) by cultured solution untrition in greenhouse in early season of 1994. YR breeded by Oryza+Sorghum with the distant pollen mutation, and show stronger tolerance to excess Fe 2+ at seedling stage than GR, which is an another intergeneric hybrid of Oryza+Zea DNA(directly introduced). In the present paper,the mechanisms of tolerance to excess Fe 2+ for the various genotype of rice varieties are discussed. There is a relationship between Fe content and K,Ca,Mg,Mn,Si,Zn,Cu content in different organs of rice plant. The effluence amount of K,Si and Mg in plant body can improve to tolerance the toxicity occured by more content of Fe 2+ in solution.