高效发光二级管光生物反应器的研制及光合自养螺旋藻的培养

The Studies on LightEmitting Diodes (LED) Panel Photobioreactor and Mass Caltivation of Spirulina. platensis

采用红光ＧａＡｌＡｓＨＢ－ＬＥＤ集成光电板作为内部光源，设计研制了６Ｌ光电板式光生物反应器，并建立了恒容连续培养体系．通过控制不同稀释率Ｄ的培养，确定Ｄ为０．１５６ｄ－１，可使藻的生物量浓度稳定在４．９８ｇＬ－１；光源品质及光照强度对螺旋藻的光合自养生长及光合色素的影响的研究表明，在ＧａＡｌＡｓＨＢ－ＬＥＤ红光培养条件下，螺旋藻生长快，干物质积累多，每克干物质量的藻含捕光色素藻胆蛋白基本稳定在１３０ｍｇ．研究结果表明，ＨＢ－ＬＥＤ具有高光照强度、低功耗的特点，对于新型光生物反应器的开发研制。

The research maily involves with desiging and developing a photobioreactor (6 L) that utilizes red GaAlAs HBLED integrated circuit block as internal illuminant and establishing the continuous system of cultivation. The biomass concentration of spirulina is steady about 498 g·L-1 when the optimal dilution rate D determined to 015 d-1by controlling fresh nutrient medium supplied. Under observation effect of the light quality and light intensity on growth of photoautotrophetic Spirulina and concentration of photosynthesis pigment, the studies indicate that high photosynthetic effciency of Spirulina, high growth rate, more dring weight of algae and the content of phycobiliprotein about 130 mg/g. DW in the condition of red light and certain range of light intensity are achieved. These results suggest GaAlAs HBLED being the most potential economic light resource for photobioreactor of development in the future. 