两种新型农药对斜生栅藻(Scenedesmus obliquus Kütz.)的毒性研究

Toxicity of Two Newly-synthesized Compounds on Scenedesmus obliquus kütz.

LY-04和WSQIF是由华中师范大学农药所合成的两种新型有机磷农药,LY-04属于a-氧代膦酸衍生物,WSQIF属于芳氧基乙酰氧基烃基膦酸脂。选用斜生栅藻(ScenedesmusobliquusKütz.)作为实验生物,通过显微计数测定藻细胞的密度以计算LY-04和WSQIF对斜生栅藻24h、48h、72h、96h的半效应抑制浓度(EC_(50)),研究其对斜生栅藻的毒性效应。结果表明:以丙酮为溶剂时,每隔24h取样,测得LY-04对斜生栅藻的48h、72h、96h的EC_(50)值分别为2942.5mg/L、239.7mg/L和65.0mg/L,而WSQIF的24h、48h、72h、96h的EC_(50)值分别为15.9mg/L、53.0mg/L、98.0mg/L和28.8mg/L。LY-04和WSQIF分别作为杀虫剂和除草剂而研制,经活体研究表明,LY-04具有较强的杀虫活性,WSQIF则具有很强的除草活性,所得EC_(50)值基本符合设计结果。且根据毒性分级标准,LY-04和WSQIF的EC_(50)值远大于3mg/L,其对非靶生物中的水生藻类都属于低毒,可推广应用。

LY-04 and WSQIF are newly-synthesized compounds which were presented by the Institute of Pesticides Chemistry,Central China Normal University.LY-04 belongs to α-ox- phosphonic acid derivatives,while WSQIF is an aromatic acetyl oxophosphonic acid derivative.In is study,the toxicity of LY-04 and WSQIF on Scenedesmus obliquus Kütz was examined.The EC50 of LY-04 and WSQIF were determined in S.obliquus cells at 24h,48h,72h,96h repre- sented by cell density.Our data showed that the 24h,48h,72h,96h EC50 of LY-04 were 1982. 5mg/L,2942.5 mg/L,239.7 mg/L and 65.0 mg/L acetone,respectively,and the 24h, 48h,72h,96h EC50 of WSQIF were 15.9mg/L,53.0 mg/L,98.0 mg/L and 28.8 mg/L respectively.These studies showed that LY-04 had higher insecticidal activity,while WSQIF had higher phytocidal activity.As LY-04 and WSQIF were designed for insecticide and herbicide re- spectively,the results were in compete with the anticipated ones.According to the toxicant classifi- cation standard,EC50 of LY-04 and WSQIF were all far higher than 3mg/L,therefore the toxici- ty of LY-04 and WSQIF to the aquatic algae were low,indicating that these two pesticides are rather safe to the aquatic ecosystem.