扶正抗癌方抑制人肺癌细胞增殖及增强LAK抑瘤活性的实验研究

Experimental Resarch of the Surpression in the Proliferation of Human Pulmonary Cell and the Improvement in Anticcer Activity of LAK Cell of Fuzhengkangaifang

研究中药复方制剂扶正抗癌方对人肺癌细胞ＬＡ、肺癌原代细胞增殖的影响，探讨其抗癌作用机制．扶正抗癌方灌胃给药后，采集大鼠血清，处理人肺癌细胞ＬＡ、人肺癌原代细胞，ＭＴＴ法观察细胞增殖；并与淋巴因子激活的杀伤细胞（ＬＡＫ）共同处理以上２种细胞．扶正抗癌方具有显著抑制人肺癌细胞ＬＡ、人肺癌原代细胞增殖的作用，且存在不同浓度之间的差异，提示呈现出血清浓度依赖性；可以显著提高ＬＡＫ抑制人肺癌细胞ＬＡ、人肺癌原代细胞增殖的活性抑制人肺癌细胞增殖，提高ＬＡＫ活性可能是扶正抗癌方抗癌的主要作用机制．

The intention is to observe the influence of fuzhengkangaifang(a kind of traditional Chicnese medicine) in the proliferation of human pulmonary carcinoma cell LA and cell, so as to discuss.its anticancer mechanisms. The method applied is to obtain tile blood serum of the rats douched with the drug, then mix the serum with human pulmoonary carcinoma cell LA and primary culture cell and finally observe their proliferation by MTT. Furthermore these two kinds of cells are treated together with LAK cells The outcome is that Fuzheogkangaifang can suppress the proliferation of human pulmonary carci- noma cell LA and primary culture cell markedly, which indicates a serum-concentration dependence to which extent the cells are suppressed. The conclusion is that the main pharmacologic mechanisms of Fuzhangkangaifang are perhaps the suppression of the proliferation of human pulmonary carcinoma cell LA and primary culture cell and the activation of LAK cells.