末端氧化途径对小麦芽中的BADH基因表达的调节(英文)

Regulation of Terminal Oxidative Pathway on BADH Gene Expression in Wheat Seedlings

KCN ( 2mol/L) ,antimycinA ( 2 .7μmol/L) ,NaN3( 6mmol/L)和SHAM (salicylhydrox amicacid ,5mmol/L)分别降低小麦芽中的BADH基因表达约 40 % ,41 % ,46%和 2 2 .0 % ,而ATP ,ADP分别增加BADH基因表达约 33%和 34% ,DNP ( 2 ,4 dinitrophenol,1mmol/L)降低BADH基因表达约 30 %。这些结果表明 ,正常运转的末端氧化途径所形成的高能化合物对BADH基因表达是重要和必需的 ,细胞色素途径比交替途径对BADH基因表达有更大的影响。这是首次对“多条路线”

Expression of the BADH (betaine aldehyde dehydrogenase) gene in the wheat seedlings lowered after treatments with KCN (2 mmol/L), antimycin A (2.7 μmol/L), NaN 3 (6 mmol/L) and salicylhydroxamic acid (SHAM, 5 mmol/L) about by 40%,41%,46% and 22% respectively. The expression increased after treatments with ATP (0.36 mmol/L) and ADP (0.46 mmol/L) about by 33% and 34% respectively, and DNP (2,4 dinitrophenol, 1 mmol/L) lowered the expression about by 30%. These results suggested the bio energy formed from the running terminal electron transport pathways would be important and necessary to the expression of the gene, and the influence of cytochrome pathway on the expression of the BADH gene was much stronger than that of alternative pathway. This is the first report to provide direct evidence to Tang′s hypothesis on multiple respiratory metabolic pathways supposing the idea that expression of plant gene was also regulated by respiratory metabolism.