摘要
应用抗人酶结合物的ELISA方法鉴定中华按蚊蚊胃人血,其结果全部呈阳性反应;而蚊胃中兔、猪和豚鼠血均呈阴性反应。蚊胃人血经2、6、12或24h的消化,仍能鉴定。结果表明ELISA鉴定中华按蚊蚊胃人血是一种敏感与特异的试验方法。
The microplate method of the ELISA was manipulated using HRP antihuman IgG conjugate for identification of Anopheles Sinensis human bloodmeals. There was all positive reaction in human bloodmeals of A.Sinensis. But negative reaction was observed in rabbit, pig and guinea-pig bloodmeals of A. Sinensis. Human bloodmeals of A. Sinensis were digested for 2,6,12, or 24 hours were yet identificated. The results showed that ELISA is a sensitive and specific test for the identification of A. Sinensis bloodmeals, also is a new technique for epidemiological studies of bloodsucking habits of A. Sinensis.
