摘要
应用细胞培养方法和核酸杂交试验从1988年1月份上海市各区搜集及同年3月份启东产地采集的毛蚶混合浓缩标本中均分离到甲肝病毒(HAV)和检测到 HAV RNA,表明启东产地的毛蚶确污染有 HAV。根据研究中核酸杂交试验的敏感性估算每只毛蚶约污染有2～8 TCID_(50)的 HAV。进一步证实了1988年春季上海地区的甲肝大流行与摄食不洁毛蚶有关。
HAV and HAV RNA were detected in Mixed, concentiated hairy-dams samples which collected from various Markets in Shanghai as well as the Nafive area in Chidong county,Jiang Su Province in Janu- ary,March 1988.The incubation period of appear- ance of HAAg in tissue-cultures was 40—60 days. Further idenfification of HAV was carried out by Immunofluorescence blocking test,Neutralization assay and Immunoelecfromicroscopy with Paired sera of chimpanzees.HAV cDNA 3831 clone ond PBR 322 DNA were used as probes for hybridization as- say.Results showed all clom samples gave positive bands with 3831 clone,but Not with pBR 322 DNA. According to the Sensitivity of hybsidization assay, the contamination rate of 2-8 TCID<sub>50</sub>of HAV in per hairy-clom was estimated.
