摘要
为探讨ＰＣＲ 检测技术在霍乱监测中的应用价值，应用ＰＣＲ 技术与培养法平行检测各类标本８６４ 份，结果ＰＣＲ 检出阳性率为７．４１ ％ ，明显高于培养法的４．５１ ％ （χ２ ＝ ５．９５ ，Ｐ＝ ０．０１４７ ＜ ０．０５） 。其中健康接触者ＰＣＲ检出率为１３．７３ ％ ，明显高于培养法的１．９６ ％ （χ２ ＝ ４．８８ ，Ｐ＜ ０．０５） ；水标本ＰＣＲ检出率９．２３ ％ ，也明显高于培养法的４．７６ ％ （χ２ ＝ ４．４８ ，Ｐ＜ ０．０５） 。表明ＰＣＲ 法既特异又敏感、快速，在霍乱监测中有很好的应用前景。
In order to evaluate the applying value of PCR in cholera sureveillance we had detected 864 sampsles with PCR and culture method parallely for cholera surveillance in 1996 to 1999.The result showed that the positive rate fo PCR was 7\^41%.It was ob viously higher than that of culture method,4\^51%(χ\+2=5\^95,P<0 05).In which,the positive rate of healthy contact with PCR was 13\^73% and significantly higher than 1\^96% of culture method.(χ\+2=4 88,P<0 05).The detecting positive rate of water samples with PCR was 9\^23% and was higher than that (4\^76%) of culture method (χ\+2=4 48,P<0 05).The result indicated that PCR was a specific、sensitive and rapid method.PCR had a good future in cholera surveillance.In some units where was good condition PCR method could be applied and spread.
基金
海南省自然科学基金
关键词
PCR
霍乱
监测
Cholera Surveillance PCR
