摘要
应用ＭＴＴ 比色、琼脂糖凝胶电泳方法研究氯化镉对小鼠腹腔巨噬细胞（ ＭΦ） 的毒性及ＤＮＡ 的损伤作用。结果表明，染毒６ 小时，氯化镉对ＭΦ活性产生抑制效应，染毒２４ 小时，抑制效应最大；镉浓度与ＭΦ活性抑制率之间存在剂量反应关系。染毒２４ 小时，镉浓度为１０ 、２０ 、４０ 、８０μｍｏｌ／Ｌ时，ＭΦＤＮＡ 电泳呈现“梯子”条带（ 凋亡特征） ；镉浓度为１６０ 、３２０μｍｏｌ／Ｌ 时，ＤＮＡ 电泳呈现“膜”状（ 坏死特征） 。本研究提示，氯化镉可抑制离体ＭΦ的活性，而且可引起ＤＮＡ 的降解，不同浓度的氧化镉引起ＭΦ损伤的方式不同。
The cytotoxicity and DNA damage of CdCl\-2 with macrophage(MΦ) by MTT method and agarose gel electrophoresis were studied.The results showed that an increase of inhibition rate of macrophage activity was detectable after 6h of intoxication with CdCl\-2,reach a maximm at 24h.This increase was dose dependent on cadmium concentration.Inspection of DNA from cells treated with cadmium for 24h reveals that at 10,20,40,80μmol/L groups produced significant DNA fragments,which represent hallmarks of apoptosis.By contrast,when macrophages were exposed to higher cadmium concentrations (160 and 320μmol/L),cell death increased with DNA film formation,which represent hallmarks of necrosis.The research inferred that CdCl\-2 could inhabit the activity of isolated mouse MΦ and induce DNA degradation.Cadmium of different concentrations kills MΦ by different ways.
