摘要
以编码甲肝病毒ＶＰ３ 羧基端的基因区为目标区，运用地高辛标记ＲＴＰＣＲ 法对甲肝疫苗进行检测，并与ＴＣＩＤ５０ 检测法进行比较。建立甲肝减毒活疫苗滴度的地高辛标记ＲＴＰＣＲ 检测法。结果显示：地高辛ＲＴＰＣＲ 法灵敏度略高于ＴＣＩＤ５０ 法，特异性与ＴＣＩＤ５０ 相似，且快速简便，易于推广应用。结果表明：地高辛标记ＲＴＰＣＲ 法是一种简便、快速、灵敏的甲肝病毒检测方法，可用作疫苗的常规检测。
Objective A highly rapid、sensitive and specific Digoxigenin labling RT PCR method for evaluation of the titre of HAV attenuated live vaccine was established.Method:Using Digoxigenin labeling RT PCR technique,the titre of HAV vaccine was tested.A highly conserved region encoding the carboxyl terminus of the capsid protein VP\-3 was selected for amplification.And the method was compared with TCID\-\{50\}.Result:The sensitivity of digoxigenin labeling RT PCR were high than TCID\-\{50\}.The specificity were similar.Conclusion:Digoxigenin labeling RT PCR is a sensitive、specific and reliable method for detection of HAV vaccine.
