摘要
用放射免疫方法检测不同期别的煤工尘肺（ＣＷＰ）患者血浆中前列腺素Ｅ２（ＰＧＥ２）的水平，同时用体外细胞培养方法将煤尘作用于肺泡巨噬细胞（ＡＭ）后对其分泌ＰＧＥ２的情况进行比较。结果表明：ＣＷＰ患者血浆中ＰＧＥ２的水平明显高于对照组；不同期别的病人血浆中ＰＧＥ２含量不一样．表现为Ⅰ＞Ⅱ＞Ⅲ，经统计分析．Ｐ＜０．０１．体外实验显示随着煤尘对ＡＭ作用浓度增加．培养时间延长，ＡＭ培养上清液中ＰＧＥ２含量升高。体内、体外的研究结果均证实煤尘作用于ＡＭ后，可以改变ＡＭ的分泌功能，使其分泌大量的ＰＧＥ２．提示：ＣＷＰ的发生发展与血浆中ＰＧＥ２的水平有一定的关系。
The contents of prostaglandin E2(PGE2) in theplasma of patients with different categorie of coal worker’S pneumocotois (CWP) and in the supernatant of alveolar macropbages(AM) culture from rats reacted with coal dust were measured by the radioimmunity assay.The results showed that the average levels of PGE2 in the plasma of CWP patients was significantly higher than that in the control group.The levels o f PGE2 in the plasma of CAP patients in different categories were different.The level of PGE2 in the plasma of CWP patients in the first categories was higher than that of in the second,and that of the second was higherthan the third.Statistics analysis showed P<0.01.The results of in vitro tests showed that the longer the culturing time and the higher the concentration in the coal dusts, the higher the PGE2 Concentration in the surpernatant.This study also showed that after the AM reacted with the coal dust,the AM secreted a huge amount of PGE2.It pointed out that the level of PGE2 in the plasma with CWP patients may be related with the occurence and development of CWP.
