摘要
采用荧光偏振及ＥＳＲ（电子自旋共振）技术观察铅对心肌细胞膜脂、膜蛋白运动的影响。与对照组相比，１～１００μｍｏｌ／Ｌ铅可使荧光偏振度Ｐ值及微粘度η值呈增加趋势，但无显著性差异。在同一剂量相同作用时间下，铅对τＣ值有明显影响，在１～５０μｍｏｌ／Ｌ范围内呈剂量反应关系，即随铅剂量增大，τＣ值增大。τＣ增大，膜蛋白运动减慢；τＣ减小，膜蛋白运动加快。提示铅对心肌细胞膜蛋白的影响大于膜脂。
With the aim to characterize the effects of lead on membrane lipids and protein mobility of myocardial cells,the fluormetry and ESR(electron Spin resonance)were applied in this study.In dose range of 1～100μmol/L,lead elicted a gradual rise in values of both P and η which can reflect changes in lipid fluidity,but insignificantly.In the same dose range and treatment hours lead could induce a increase in the value of τ C in a dose dependent manner.The higher the value of τ C,the slower the mobility of protein.In combination with the related results,the findings suggested that the membrane protein was vulnerable to the effect of lead,rather than the membrane lipids.
基金
国家教委博士点基金
国家自然基金
