摘要
Despite extensive investigation, the pathophysiology of human inflammatory bo wel disease (IBD) remains incompletely understood. We examined the existence of oxidative and nitrosative stress and also alterations in epidermal growth factor (EGF) secretion in saliva of IBD patients. Saliva samples were obtained from 30 nonsmoking IBD patients including 16 Crohn’ s disease (CD) pat ients and 16 ulcerative colitis (UC) patients and 16 age- and sex- matched co ntrols. Samples were analyzed for thiobarbituric reactive substances (TBARS) as a marker of lipid peroxidation, ferric reducing ability (antioxidant power), and EGF and nitric oxide (NO) levels. Saliva TBARS levels increased significantly ( P < 0.01) in CD patients but not in UC patients. Analysis of antioxidant power r evealed that saliva of CD patients has lower antioxidant power (P < 0.01) than s aliva of the healthy control population. The concentration of EGF was found to b e increased (P < 0.01) in saliva of CD patients in comparison to that of healthy subjects. NO levels increased in saliva of both CD and UC patients in compariso n to that of healthy subjects. It is concluded that excessive NO production is p resent in saliva of both CD and UC patients but only saliva of CD patients is ox idatively stressed. EGF secretion is normal in UC patients, although CD patients show a significant increase in salivary EGF levels.
Despite extensive investigation, the pathophysiology of human inflammatory bo wel disease (IBD) remains incompletely understood. We examined the existence of oxidative and nitrosative stress and also alterations in epidermal growth factor (EGF) secretion in saliva of IBD patients. Saliva samples were obtained from 30 nonsmoking IBD patients including 16 Crohn' s disease (CD) pat ients and 16 ulcerative colitis (UC) patients and 16 age- and sex- matched co ntrols. Samples were analyzed for thiobarbituric reactive substances (TBARS) as a marker of lipid peroxidation, ferric reducing ability (antioxidant power), and EGF and nitric oxide (NO) levels. Saliva TBARS levels increased significantly ( P < 0.01) in CD patients but not in UC patients. Analysis of antioxidant power r evealed that saliva of CD patients has lower antioxidant power (P < 0.01) than s aliva of the healthy control population. The concentration of EGF was found to b e increased (P < 0.01) in saliva of CD patients in comparison to that of healthy subjects. NO levels increased in saliva of both CD and UC patients in compariso n to that of healthy subjects. It is concluded that excessive NO production is p resent in saliva of both CD and UC patients but only saliva of CD patients is ox idatively stressed. EGF secretion is normal in UC patients, although CD patients show a significant increase in salivary EGF levels.
