葛根异黄酮对地塞米松致大鼠骨质疏松症的保护作用

Protective Effect of Total Isoflavones from Pueraria lobata on Secondary Osteoporosis Induced by Dexamethasone in Rats

目的:研究葛根总异黄酮(TIP)对皮质激素(地塞米松)引起的继发性骨质疏松症的防治作用。方法:4月龄SD大鼠,每周2次肌肉注射磷酸地塞米松1. 5 mg/kg,并口服TIP 100、50和25 mg/kg,同时设VD阳性对照组。在3和6个月时测定大鼠全身骨矿总量(BMC)的骨矿密度(BMD),6个月时测股骨生物力学性能、比重、灰重和钙含量等。结果:(1)TIP高剂量组(100 mg/kg)3和6个月BMD增加3.4％和8.4％,BMC增加7.0％和9.1％;中剂量组(50 mg/kg)使BMD增加3.2％和5.3％,BMC增加6.2％和6.0％。(2)TIP高剂量组股骨最大负荷和结构强度分别提高10.3％和10.5％,TIP中剂量组分别提高3.5％和7.1％。(3)TIP高剂量组和中剂量组轻微增加股骨和胫骨湿重和干重,但明显增加湿比重(+2.7％和+2.0％)与干比重(+2.7％和2.6％)。(4)TIP高剂量组使单位体积股骨内灰重和钙含量增加3.1％和4.2％,TIP中剂量组增加4.3％和6.4％。TIP低剂量组(25mg/kg)各指标变化不明显。结论:TIP对地塞米松致大鼠继发性骨质疏松症具有明显的防治作用。

Objective:To study the protective effect of the total isoflavones from Pueraria lobata (TIP) on secondary osteoporosis induced by dexamethasome (DXM). Methods: Female SD rats,4 month aged, were given intramuscularly with 1.5 mg/kg DXM twice once week.The treated groups were orally given TIP 100, 50 or 25 mg·kg-1·d-1 for 6 months.The whole body BMD and BMC were detected at 3 and 6 months.The biodynamics potency, density, ash weight and calcium content of femur were determined at 6 months.Results: (1) At 3 and 6 months, BMDs in TIP 100 mg/kg group were increased by 3.4% and 8.4% than that in model group, BMC were increased by 7.0% and 9.1%, while in TIP 50 mg/kg group by 3.2%, 5.3% and 6.2% ,6.0% .(2) The Fmax and hardness in TIP 100 mg/kg group were enhanced by 10.3% and 10.5% , when in TIP 50 mg/kg group were enhanced by 3.5% and 7.1% . (3)TIP can increase wet and dry weight in slightly extent,but significantly increase wet density and dry density. (4)The ash weight and calcium content in femur were increased by 3.1% and 4.2% in TIP 100 mg/kg group,4.3% and 6.4% in TIP 50 mg/kg group, while no change in TIP 25 mg/kg group. Conclusions: TIP can prevent secondary osteoporosis induced by DXM in rats.