HPLC法测定天泉通颗粒中芍药苷的含量

HPLC determination of paeoniflorinin in Tianquantong granules

目的:建立HPLC法测定天泉通颗粒中芍药苷的含量。方法:采用Hypersil C18柱(150mm×4.6mm,5μm),流动相为甲醇-水(30∶70),紫外检测波长为230nm。结果:芍药苷进样量在0.201~3.22μg范围内与峰面积线性关系良好,平均加样回收率为99.1%,RSD为2.0%(n=9)。结论:本法简便,测定结果准确、重现性好,可控制天泉通颗粒的质量。

Objective:To establish a HPLC method for determination of Paeoniflorinin in Tianquantong granules.Methods:This system consisted of Hypersil C18 column(150mm×4.6mm,5μm)and methanol-water(30∶70)was used as mobile phase,with the detection wavelength at 230 nm.Results:There was a good linear correlation with paeoniflorinin between 0.201～3.22μg.The average recovery rate of paeoniflorinin was 99.1% and RSD was 2.0%(n=9).Conclusion:The method is simple,accurate,reproducible and can be used for the quality control of paeoniflorinin in Tianquantong granules.