脑脉宝对大鼠脑缺血再灌注损伤的保护作用

Protective Effects of Naomaibao(脑脉宝) on Cerebral Ischemia Reperfusion Injury in Rats

为了探讨脑脉宝对大鼠脑缺血再灌注损伤的保护作用及机理。采用四血管阻断法造成大鼠脑缺血30min 再灌注30min。结果中见到脑组织中去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、钾和镁含量明显降低(P<0.01～0.05),过氧化脂质(LPO)、脑含水量、钙含量显著升高(P<0.05)。脑神经病理和超微结构均出现了严重的缺血性损害,可见大量 Ca^(2+)沉淀,皮层及海马中出现大量 c-fos 蛋白阳性表达产物。预先给予脑脉宝可升高 NE、DA、5-HT 含量及 SOD、GSH-Px 活性(P<0.001～0.05),降低 LPO、脑含水量、钙含量和脑指数(P<0.01～0.05),明显减轻缺血性脑损害,减少胞内钙积聚,部分抑制 c-fos 基因异常表达。认为该药可通过抑制再灌注后自由基引起的脂质过氧化反应,提高抗氧化酶活性,改善单胺类神经递质代谢紊乱及离子紊乱、脑组织水钠代谢异常,阻止 Ca^(2+)内流,抑制 c-fos 原癌基因异常表达,从而提高神经组织对缺血缺氧的耐受性,减轻脑水肿和脑缺血性损害,促进神经功能恢复。

This study was designed to investigate the effects of Naomaibao（NMB）on cerebral ischemia reperfusion injury in rats.The results showed that in model rat,the contents of norepinephrine（NE）,dopamine（DA）and serotonin（5-HT）,activities dr super- oxidedismutase（SOD）and glutathione peroxidase（GSH-Px）,concentrations of magnesium(Mg2+)and potassium（K+） markedly reduced（P<0.01～0.05）,lipid peroxides（LPO）,the brain water and calcium(Ca2+)significandy increased（P< 0.01～0.05）,neuropathology and ultrastructure severe ischemic damages took place,large Ca2+deposits accumulated in cells, significant presence of c-fos protein in cortex and hippoeampus appeared.Pretreatment with NMB could inhibit the reduction of NE,DA,5-HT,SOD,GSH-Px activities（P<0.01～0.05）and elevation of LPO,water,Ca2+and brain index（P<0.01～ 0.05）,substantially reduce the ischemic damages,diminish the ultrastructural abnormalities and intracellular Ca2+accumla- tion,inhibit the expression of c-fos protein.The observation suggested that NMB could increase the animal’s tolerance to cere- bral ischemia and hypoxia,alleviate cerebral edema and ischemie damages,promte the recovery of nervous function through suppressing reperfusion-induced lipid peroxidation caused by oxygen free radicals,improving the monoamine neurotransmitters disturbances,ion derangments and the metabolisms of H2O and sodium,preventing Ca2+influx,inhibiting the expression of c- fos protein after ischemia reperfusion.