黄芪甙对体外缺氧培养的人肺动脉平滑肌细胞增殖和胶原分泌的影响

Effect of Astragaloside on Proliferation and Collagen Secretion of Pulmonary Artery Smooth Muscle Cells by Hypoxia

应用体外培养肺动脉平滑肌细胞(PASMC)并缺氧的方法,分别掺入~3H-TdR(胸腺嘧啶核苷)、~3H-脯氨酸,探讨中药制剂黄芪甙对缺氧培养的 PASMC 生长分泌的影响。细胞分常氧、缺氧两种方式培养,每种培养方式又分为黄芪甙组与正常对照组。无论缺氧与常氧,黄芪甙组与正常对照组相比,~3H-TdR 掺入值(反映 DNA 的合成即细胞增殖)都有显著下降,分别为常氧:每分钟液闪值(CPM 值)由2874.50±401.60下降到365.40±78.64,缺氧:CPM 值由5956.67±1977.49下降到1002.67±508.52(P【0.01)。黄芪甙同时抑制常氧、缺氧体外培养的 PASMC ~3H-脯氨酸掺入(反映胶原的合成、分泌),分别为常氧:CPM 值由3088.70±988.80下降到1977.60±911.97,缺氧:4714.93±1802.53下降到3180.50±1790.90(P【0.05),经两因素随机取样方差分析用药与缺氧两因素间交互影响显著。提示黄芪甙可抑制体外培养的PASMC 增殖及胶原分泌,黄芪甙尤其对缺氧引起的增殖分泌效应抑制作用更明显。

Cell culture technique was applied for detection of the effect of astragaloside in proliferation and collagen secretion of pul- rnonary artery smooth muscle cells (PASMC) by hypoxia.The incorporation of ~3H-TdR with the use of astragaloside decreased from 2874.5±401.6 to 365.4±78.64 in normal condition and from 5956.67±1977.49 to 1002.67±508.52 in hypoxia.The incorporation of ~3H-Prolin declined from 3088.7±988.8 to 1977,6±911.97 in normal condition,and from 4714.93±1802. 53 to 3180.5±1790.9 in hypoxia.It indicated that astragaloside could inhibit the proliferation and collagen secretion of PASMC in both hypoxia and normaxia,especially in hypoxia.