抗人膀胱癌双功能抗体对荷瘤鼠的免疫治疗

The immunotherapeutic effect of bifunctional antibody on mice borne human bladder cancer

目的 :建立抗人膀胱癌双功能抗体对荷人膀胱癌小鼠主动免疫的模型。方法 :采用抗人膀胱癌双功能抗体免疫 BALB/C小鼠 ,对照组采用生理盐水。免疫 3次后 ,于末次免疫后 1周 ,将新鲜的人膀胱移行细胞癌组织移植于小鼠肾包膜下 ,移植后第 2、4、6、8和 1 0天处死小鼠 ,取血清进行抗双功能抗体分析。移植瘤行组织学检查 ,观察宿主淋巴细胞浸润情况及瘤细胞可见率。结果 :实验组小鼠肾脏包膜下人膀胱癌细胞很快受到排斥 ,在移植后第 6天 ,淋巴细胞浸润即达高峰 ,而对照组在第 1 0天淋巴细胞浸润才达高峰 ;实验组在移植后第 6天瘤细胞可见率即明显降低 ,而对照组呈逐渐下降趋势 ;实验组抗双功能抗体为阳性 ,对照组为阴性。结论 :抗人膀胱癌双功能抗体作为抗原免疫小鼠后 ,能够排斥小鼠体内人膀胱癌细胞的生长。

Objective:To establish a immunotherapeutic model of human ladder cancer in mice to test effect of bifunctional antibody (BfAb) on the tumor. Methods:BALB/C mice were immunized once a week for 3 times with the BfAb. The control group were injected with salt.Fresh bladder tumor cells were implanted into the renal subcapsule of immunized mice after the last immunization. The mice then were killed 2 nd ,4 th ,6 th ,8 th ,and 10 th days later, respectively,after the transplantation. BfAb in sera was detected by ELISA inhibition assay. Tumor infiltration lymphocytes and tumor cells from the tumor tissue of mice were observed under microscope.Results:anti BfAb level in the sera of immunized mice was higher than that of the controls. Tumor infiltration lymphocytes in the former groups were found to reach their peak in the 6 th day,whereas the highest peak in the control group was found in the 8 th ~10 th day;tumor cell viability rate in the former was decreased rapidly after 6 days,but decreased gradually in the control. Conclusion:BfAb could prevent growth of human bladder cancer in mice after mice were immunized.