雷公藤单体体外抑制胶质瘤细胞的实验研究

Several Monomes from Tripterygium Wilfordii Inhibit Proliferation of Glioma Cells In Vitro

背景与目的:已有研究发现雷公藤具有抗肿瘤作用,本研究旨在探讨雷公藤单体对胶质瘤细胞的体外抑制作用。方法:通过MTT法测定3种雷公藤单体(甲素、红素和WilforolA)对胶质瘤细胞株SHG44、C6、U25的体外抑制作用;应用免疫组化法观察雷公藤甲素与雷公藤红素对SHG44胶质瘤细胞中Bax、Bcl-2蛋白表达的影响。结果:雷公藤二萜类单体雷公藤甲素对胶质瘤细胞有极显著的抑制作用;雷公藤三萜类单体中红素的抑制作用次之。两者均使SHG44细胞Bax表达增加,Bcl-2表达下降。结论:雷公藤甲素与雷公藤红素对胶质瘤细胞有明显的抗肿瘤作用,其作用与促进Bax表达、抑制Bcl-2表达、导致细胞凋亡有关。

Background and Objective: Researches indicated that Tripterygium wilfordii possess antitumor activity.The current study was designed to investigate inhibitive effect of several monomes of Tripterygium wilfordii on the proliferation of glioma cells. Methods:The effect of three monomes from Tripterygium wilfordii on the proliferation of glioma cell lines SHG44,C6,and U251 in vitro was examined by using MTT assay. Immunohistochemistry was used to examine the expression of Bax, Bcl 2 after treatment of triptolide and celastrol. Result:The proliferation of glioma cells was remarkably inhibited by triptolide and celastrol. They both increased expression of Bax and decreased expression of Bcl 2 in the SHG44 cells. Conclusion:Triptolide and celastrol inhibit the proliferation of glioma cells in vitro, which was associated with promoting the expression of Bax and inhibiting the expression of Bcl 2 and accelerating cell apotosis.