脑星形细胞肿瘤组织中内皮素-1的表达及定量分析

Endothelin-1 Expression and Quantitative Analysis in Astrocytomas

背景与目的:已有研究表明内皮素(endothelin-1,ET-1)可以在一些恶性肿瘤中合成、释放,并对肿瘤的发生、发展有重要的影响。本文研究ET-1在脑星形细胞瘤组织中的表达及含量与组织学分级的关系。方法:采用免疫组织化学S-P法及图像分析技术测定70例不同级别的星形细胞瘤组织中的ET-1表达的阳性率和含量。结果:ET-1普遍存在于星形细胞瘤,其阳性表达主要在胞浆内。Ⅳ级和Ⅲ级星形细胞瘤ET-1阳性率(86.67%和93.33%)与阳性细胞中的ET-1含量(0.1875±0.0227和0.1516±0.0134)都明显高于Ⅱ级(75.00%;0.1215±0.0116)和Ⅰ级星形细胞瘤(66.67%;0.1048±0.0143)及正常星形细胞(37.50%;0.0717±0.0074)(P<0.05,P<0.01)。分析表明:肿瘤分化程度越低,则ET-1的阳性率和含量越高,肿瘤分化程度越高,则ET-1的阳性率和含量越低。ET-1含量与肿瘤的分化程度呈正相关(r=0.863,P<0.01)。结论:ET-1可作为星形细胞瘤恶性生长的一个指标。

Background & Objective: It is demonstrated that endothelin 1 (ET 1) is synthesized by and released from certain malignant tumor cells, and it plays an important role in growth and development of tumor. This study was designed to investigate the expression of ET 1 in astrocytomas and the relationship between the ET 1 quantity and the grades of astrocytomas. Method: ET 1 expression was determined in 70 astrocytoma specimens using Streptavidin Peroxidase method and image analysis technology. Result: The ET 1 expressed in all of astrocytomas, and the ET 1 expression was mainly located in the cytoplasm. The positive rates of ET 1 in grade Ⅳand Ⅲ astrocytomas (86.67% and 93.33%)were significantly higher than that in grade Ⅱ,Ⅰ and normal astrocytes(75.00%,66.67%,and 37.50%)(P< 0.05). The results of image analysis on astrocytoma (grade Ⅳ,Ⅲ,Ⅱ,Ⅰ and normal control:0.1875±0.0227,0.1516±0.0134, 0.1215±0.0116,0.1048±0.0143,and 0.0717±0.0074,respectively) showed that the lower differentiation of astrocytoma,the higher ET 1 expression (P< 0.01). The expression of ET 1 was significantly correleted with the tumor grading (r=0.863,P< 0.01). Conclusion: The ET 1 quantitative analysis may be used as a monitoring index of astrocytoma growing.