凋亡调节基因在Ⅲ、Ⅳ期子宫内膜异位症中的表达

Immunolocalization of the Apoptosis Regulating Proteins in Ectopic Endometrium from Women with Advanced Endometriosis

目的 :检测凋亡调节基因 bcl- 2、bax和 c- myc在 、 子宫内膜异位症异位内膜中的表达 ,以探讨凋亡调节基因在子宫内膜异位症发病中的作用。方法 :2 0例子宫内膜异位症患者 ,按 r AFS分期 : 期 8例 , 期 12例。用免疫组化法检测上述患者异位内膜中 bcl- 2、bax和 c- m yc的表达。结果 :bcl- 2、bax和 c- myc主要定位于子宫内膜异位症的腺上皮中 ,其中 bcl- 2的总体阳性率为 5 5 % ,bax的阳性率为 75 % ,c- myc阳性率为 5 5 % ,且 bcl- 2的表达与 c- myc表达正相关(P<0 .0 1) ,而与 bax的表达负相关 (P<0 .0 5 ) ,相关性有统计学意义。按疾病分期比较三者的表达情况 ,bcl- 2在 期中表达高于 期者 ,差异有显著性 (P<0 .0 5 ) ;按月经周期比较三者表达情况 ,则差异无显著性 (P>0 .0 5 )。结论 :子宫内膜异位症患者异位内膜存在一定程度凋亡 , 期较 期细胞更易凋亡 ,而与月经周期无关 ,bcl- 2和 bax起主要调节作用 ,c- myc可能与 bcl-

Objectives:To evaluate bcl 2,bax and c myc expression in ectopic endometrium from women with advanced endometriosis.Methods: Women with untreated endometriosis(n=20)were divided into StageⅢ(n=8)and Stage Ⅳ(n=12)according to the revised American Fertility Society scoring system.Ectopic endometrium samples were collected during operation.Bcl 2,bax and c myc expressions were assessed with use of immunohistochemical techniques.Results: Bcl 2,bax and c myc immunoreactivity was found predominately in the glandular epithelial cells.The positive rate of bcl 2,bax and c myc was 55 %,75 % and 55 % respectively.The expression of bcl 2 had positive correlation with that of c myc( P <0.01) but negative correlation with the expression of bax ( P <0.05).In women with Stage Ⅳ endometriosis bc1 2 expression was more frequently than that was seen in women with Stage Ⅲ disease( P <0.05).No statistically significant difference were found in the expression of bcl 2,bax and c myc throughout the menstrual cycle ( P >0.05).Conclusions: Apoptosis may be implicated in the pathogenesis of endometriosis .Bcl 2 and bax expressions may be important regulators of cell proliferation in endometriotic tissue while c myc may cooperate with bcl 2.