结核病人血清TGF-β_1、IFN-γ的检测

Dectection of TGF-β_1、IFN-γ in tuberculosis patients

目的探讨转化生长因子 β1 (TGF β1 )及干扰素γ(IFN γ)在结核病免疫机制中的作用。方法采用ELISA法测定 61例结核病人TGF β1 、IFN γ水平 ,并用 1 6例正常健康人作对照。结果结核组血清TGF β1 明显高于对照组 (P <0 0 0 1 ) ,复治组高于初治组 (P <0 0 5)。结核组血清IFN γ低于对照组 (P <0 0 0 5) ,TGF β1 与IFN γ呈负相关 (r =- 0 41 5 ,P <0 0 0 1 )。结论人体感染结核杆菌后 ,TGF β1 增高 ,IFN γ减低 ,可能引起免疫功能失衡 ,是导致结核病的发病因素之一。TGF β1 越高 ,IFN γ越低 。

objective To explore the role of TGF β 1?IFN γ in the immune mechanism of human tuberculosis. Methods The level of TGF β 1?IFN γ of the serum obtained from 61 tuberculosis patients and 16 healthy adults were detected with ELISA. Results The concentration of TGF β 1 in serum in tuberculosis patients was significantly higher than that in the controls group ( P <0.001) and higher in the retreatment group than that in the first treatment group ( P <0.01). The concentration of IFN γ in serum of tuberculosis patients was lower than in controls( P <0.05). For all patients, there were a significant negative relation between the TGF β 1 and IFN γ level (r=-0.415, P <0.001). Conclusion The infection of mycobacterium tuberculosis may induce immunoregulation disturbance characteriled by the increase of TGF β 1 and decrease of IFN γ,these may be the cause which led to occurrence of tuberculosis. The higher the TGF β 1 level and the lower the IFN γlevel that the more serious the patient's condition.