秋水仙碱对免疫性肝纤维化的防治作用

Effects of Colchicine on the hepatic fibrosis induced by porcine serum in the rats

目的观察秋水仙碱对猪血清免疫性肝纤维化大鼠的影响。方法腹腔注射猪血清复制大鼠免疫性肝纤维化模型 ,ig秋水仙碱 1 2周 ,监测尿羟脯氨酸 ,实验结束后测血清生化指标、肝羟脯氨酸和丙二醛 ,做肝病理组织学检查。结果秋水仙碱改善肝细胞病变 ,减少肝细胞坏死 ,汇管区胆管周围纤维结缔组织增生减少 ,肝纤维化程度减轻 ;肝胶原蛋白和丙二醛含量显著降低 (P <0 .0 1 ) ,尿羟脯氨酸排出量显著增加 (P <0 .0 1 ) ;谷丙转氨酶和碱性磷酸酶的活力显著降低 (P <0 .0 5 ,0 .0 1 ) ;肝脏指数显著减小 (P <0 .0 5) ;总蛋白、白蛋白和白球比值明显升高 (P <0 .0 5 ,0 .0 1 )。结论秋水仙碱具有抑制脂质过氧化 ,促进胶原降解 。

objective To investigate the preventive actions of colchicine on the hepatic fibrosis induced by procine serum. Methods Rats were repeated injection of porcine serum intraperitoneally 0.5ml, twice a week for 12 weeks. Colchicine groups were administrated Colchicine. The urinary hydroxyproline was detected every 3 weeks. Rats were killed at the end of the 12nd week. Livers were fixed and examined by light microscopy. The serum of each rat was measured the level of total protein(TP), alkaline phosphatase(AKP), alanine aminotransferase(ALT), albumin(ALB),albumin/globulin(A/G). Hydroxyproline (Hyp),lipid peroxidation Malondialdehyde (MDA) and liver index of liver were assayed. Results The colchicine inhibited serum ALT and AKP activities( P <0.01) , lowered liver index( P <0.05), liver collagen( P <0.01), MDA ( P <0.01) and the degree of liver fibrosis, increased urine Hyp?TP?ALB and A/G level ( P <0.05, 0.01). Conclusion Colchine can effectively prevent the immune respine of the hepatic fibrosis induced by porcine serum.