抗乳腺癌人单克隆抗体用于裸鼠移植瘤显像

APPLICATION OF HUMAN MONOCLONAL ANTIBODY TO RADIOIMMUNOIMAGING OF XENOGRAFTED BREAST CANCER

乳腺癌的发病率占恶性肿瘤的7～10％,是女性癌症致死的主要原因之一。在我国女性乳癌发病率约为23/10万人,仅次于子宫颈癌。以单克隆抗体为工具,探索乳腺癌的诊断和治疗问题,有重要临床价值。由于人体对鼠单克隆抗体产生明显排异反应,所以只有制备人单克隆抗体才能有效、安全地解决体内定位诊断和治疗的问题。为此,我们采用人鼠种间杂交骨髓瘤细胞SMH-D_(33)与人淋巴结B细胞融合,制备了人单克隆抗体CH·1(HMcAb-CM·1),进行实验研究,现报道如下。

Human anti-breast cancer monoclonal antibody CM. 1 was developed by fusing-human-mouse heteromyeloma cells SMH-D33 and human B cells isoleted from axillarymetastatic lymph nodes in Patient with breast cancer.The biodistribution of radioiodi-nated HMcAb-CM. 1 was studied by measuring parameters of specific radioreactivityof xenografted tumors in nude mice. The ratio of T/NT(tumor to non-tumor tissue)andLI(Iocalization index) were more than 2. 98 and 5.25 respectively at 144-hour. Afterintraperitoneal injection of^(131)I-HMcAb-OM. 1. into nude mice bearing human breastcancer, imaging of xenografted breast cancer became increasingly distinct with thefainting background of non-tumor tissue,especially during the period of 96 to 144hours.More than 80 cases of normal and tumor tissues were examined immunohistochemicallywith HMcAb-CM. 1. breast cancer tissues were all strongly positive as compared withthe negative normal liver, lung, stomach, breast and fibroadenoma of breast. Theresults demonstrated the possibility of clinical use of HMcAb-OM. 1 for radioimmuno-imaging of primary and metastatic breast cancer.